Objective of the study:
There is enough evidence for the carcinogenicity of HPV16 type in the head and neck cancers. This study aimed to address key information gaps of the prevalence of HPV infection in laryngeal squamous cell carcinoma in Azerbaijan population.
Materials and methods:
50 LSCC pretreatment FFPE biopsy tumor materials, 10 cervical cancer FFPE samples and 38 fresh pretreatment LSCC tumor materials were analyzed using RT-PCR technology and Anyplex II HPV28 genotyping assay. The FFPE tumor material with HPV positive history from cervical cancer was as control in this study.
DNA extraction from FFPE and fresh samples was successful for all 98 tumor materials. The results of HPV genotyping of cervical cancer patients (n=10) with Anyplex II HPV28 assay detected 8 HPV16-positive cancers, 1 samples with HPV18-positive and 1 cancer material with HPV16+HPV18+HPV58. The results were predictable, because in these patients, HPV positivity was validated by Pap smear method. Based on this, we expect to get true information about HPV-related LSCC. Anyplex IIHPV28 genotyping assay was detected only one HPV positive sample of 61 LSCC: it was low-risk cancer-related HPV54 subtype in 47 age’s patient with 20 years tobacco and alcohol consumption history. High-risk cancer related HPV16 and HPV18 subtypes have no detected in analysis patients.
Thus, we suggested that PCR techniques and Anyplex II HPV28 genotyping assay were available to detect HPVs from fresh LSCC material. Our results support us to believe that Azerbaijan belongs to HPV low-incidence geographic region of laryngeal squamous cell carcinoma patients, but large-scale population-based studies are needed to confirm our findings.
Aziz J. Aliyev, Leylakhanim A. Melikova, Elnara E. Bagirova, Kamal S. Akbarov, Aytan M. Aliyeva, Nazim A. Quliyev